Extraction Methods

iSWAB®-DNA

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Manual Extraction

  • QIAamp DNA Blood Mini Kit (Qiagen, Cat. # 51104)
  • Beckman Coulter Agencourt Genfind™ v2 Blood & Serum DNA Isolation Kit
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Automated Extraction

  • (Maxwell® RSC Blood DNA Kit, Promega
  • (Agencourt Genfind v2, Beckman Coulter, Cat. # A41501, A41499, A41497, A41500)
  • AnaPrep 12 Blood DNA Extraction Kit (BioChain, Ct. # z1322001)

 

iSWAB automated liquid handling compatible platforms

  • Perkin Elmer: chemagic MSM I
  • Qiagen: The customized protocol B400_CR21913_ID2179.xml is based on the standard protocol Blood_400_V6_DSP.xml. and is usable in combination with the QIAsymphony DSP DNA Midi Kit (96) (catalog no. 937255). Please contact Qiagen Technical Support for implementation. 
  • Beckman Coulter: Biomek, SPRI-TE
  • Hamilton Star, Hamilton Nimbus
  • Tecan Evo
  • Promega Maxwell-16 RSC, or RSC 48

iSWAB®-Discovery

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Manual Extraction

  • QIAamp DNA Blood Mini Kit (Qiagen, Cat. # 51104)
  • Beckman Coulter Agencourt Genfind™ v2 Blood & Serum DNA Isolation Kit
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Automated Extraction

  • (Maxwell® RSC Blood DNA Kit, Promega, Cat.# AS1400)
  • (Agencourt Genfind v2, Beckman Coulter, Cat. # A41501, A41499, A41497, A41500)
  • AnaPrep 12 Blood DNA Extraction Kit (BioChain, Ct. # z1322001)

 

iSWAB automated liquid handling compatible platforms

  • Perkin Elmer: chemagic MSM I
  • Qiagen: The customized protocol B400_CR21913_ID2179.xml is based on the standard protocol Blood_400_V6_DSP.xml. and is usable in combination with the QIAsymphony DSP DNA Midi Kit (96) (catalog no. 937255). Please contact Qiagen Technical Support for implementation. 
  • Beckman Coulter: Biomek, SPRI-TE
  • Hamilton Star, Hamilton Nimbus
  • Tecan Evo
  • Promega Maxwell-16 RSC, or RSC 48

iSWAB®-ID

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Manual Extraction

  • QIAamp DNA Blood Mini Kit (Qiagen, Cat. # 51104)
  • Beckman Coulter Agencourt Genfind™ v2 Blood & Serum DNA Isolation Kit
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Automated Extraction

  • (Maxwell® RSC Blood DNA Kit, Promega, Cat.# AS1400)
  • (Agencourt Genfind v2, Beckman Coulter, Cat. # A41501, A41499, A41497, A41500)
  • AnaPrep 12 Blood DNA Extraction Kit (BioChain, Ct. # z1322001)

 

iSWAB automated liquid handling compatible platforms

  • Perkin Elmer: chemagic MSM I
  • Qiagen: The customized protocol B400_CR21913_ID2179.xml is based on the standard protocol Blood_400_V6_DSP.xml. and is usable in combination with the QIAsymphony DSP DNA Midi Kit (96) (catalog no. 937255). Please contact Qiagen Technical Support for implementation. 
  • Beckman Coulter: Biomek, SPRI-TE
  • Hamilton Star, Hamilton Nimbus
  • Tecan Evo
  • Promega Maxwell-16 RSC, or RSC 48

iSWAB®-Animal

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Manual Extraction

  • ReliaPrep Blood gDNA Miniprep System (Promega Cat. A5081).
  • NEB Monarch HMW gDNA Extraction kit (NEB #T3050, #T3060)
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Automated Extraction

  • Maxwell RSC Blood DNA Kit, (Promega Cat. #AS1400).

 

iSWAB automated liquid handling compatible platforms

  • Perkin Elmer: chemagic MSM I
  • Qiagen:  The customized protocol B400_CR21913_ID2179.xml is based on the standard protocol Blood_400_V6_DSP.xml. and is usable in combination with the QIAsymphony DSP DNA Midi Kit (96) (catalog no. 937255). Please contact Qiagen Technical Support for implementation. 
  • Beckman Coulter: Biomek, SPRI-TE
  • Hamilton Star, Hamilton Nimbus
  • Tecan Evo
  • Promega Maxwell-16 RSC, or RSC 48

iSWAB®-Microbiome

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Manual Extraction

  • QIAamp MinElute Virus Spin Kit (Qiagen, Cat.# 57704)
  • QIAamp Fast DNA Stool Mini Kit (Qiagen Cat# 51604)
  • Quick-DNA Fecal/Soil Microbe Miniprep Kit (Zymo Research Cat# 6010)
  • DNeasy PowerSoil Pro Kit (Qiagen Cat#47014)
  • QIAamp PowerFecal Pro DNA Kit (Qiagen, Cat #51804
  • MegAttract PowerMicrobiome DNA/RNA EP (Qiagen, Cat#27500)
  • NucleoMag DNA Microbiome (Macherey-Nagel, Cat# 67-63-0)
  • NucleoMag DNA Microbiome for DNA purification from soil, stool, and biofilm (Macherey-Nagel, Cat# 744330.4)

 

iSWAB automated liquid handling compatible platforms

  • Perkin Elmer: chemagic MSM I
  • Beckman Coulter: Biomek, SPRI-TE
  • Hamilton Star, Hamilton Nimbus
  • Tecan Evo
  • Promega Maxwell-16 RSC, or RSC 48

iSWAB®-Microbiome FecesCatcher

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Manual Extraction

  • QIAamp MinElute Virus Spin Kit (Qiagen, Cat.# 57704)
  • QIAamp Fast DNA Stool Mini Kit (Qiagen Cat# 51604)
  • Quick-DNA Fecal/Soil Microbe Miniprep Kit (Zymo Research Cat# 6010)
  • DNeasy PowerSoil Pro Kit (Qiagen Cat#47014)
  • QIAamp PowerFecal Pro DNA Kit (Qiagen, Cat #51804
  • MegAttract PowerMicrobiome DNA/RNA EP (Qiagen, Cat#27500)
  • NucleoMag DNA Microbiome (Macherey-Nagel, Cat# 67-63-0)
  • NucleoMag DNA Microbiome for DNA purification from soil, stool, and biofilm (Macherey-Nagel, Cat# 744330.4)

 

 

iSWAB automated liquid handling compatible platforms

  • Perkin Elmer: chemagic MSM I
  • Beckman Coulter: Biomek, SPRI-TE
  • Hamilton Star, Hamilton Nimbus
  • Tecan Evo
  • Promega Maxwell-16 RSC, or RSC 48

iSWAB®-Microbiome-EL

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Manual Extraction

  • No extraction required. Direct PCR
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Automated Extraction

  • No extraction required. Direct PCR

 

iSWAB automated liquid handling compatible platforms

  • Perkin Elmer: chemagic MSM I
  • Beckman Coulter: Biomek, SPRI-TE
  • Hamilton Star, Hamilton Nimbus
  • Tecan Evo
  • Promega Maxwell-16 RSC, or RSC 48

iSWAB®-RNA-V2

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Manual Extraction

  • RNeasy Mini Kit (Qiagen, Cat. # 74104)
  • Magbio HighPrep Total RNA Plus Kit

 

iSWAB automated liquid handling compatible platforms

  • Perkin Elmer: chemagic MSM I
  • Beckman Coulter: Biomek, SPRI-TE
  • Hamilton Star, Hamilton Nimbus
  • Tecan Evo
  • Promega Maxwell-16 RSC, or RSC 48

iSWAB®-Cells

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Manual Extraction

  • RNeasy Mini Kit (Qiagen, Cat. # 74104)
  • Magbio HighPrep Total RNA Plus Kit
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Automated Extraction

  • HighPrep Total RNA Isolation Kit – iSWAB® (MagBio, Cat.# ISTOR-R50, ISTOR-R100, ISTOR-R100x4)

 

iSWAB automated liquid handling compatible platforms

  • Perkin Elmer: chemagic MSM I
  • Beckman Coulter: Biomek, SPRI-TE
  • Hamilton Star, Hamilton Nimbus
  • Tecan Evo
  • Promega Maxwell-16 RSC, or RSC 48

HemaSure

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Manual Extraction

  • QIAamp DNA Blood Mini Kit (Qiagen, Cat. # 51104)
  • QIAamp Circulating Nucleic Acid Kit (Qiagen, Cat. # 55114)
  • RNeasy Mini Kit (Qiagen, Cat. # 74104)
  • Biochain Whole Blood RNA Extraction Kit (Biochain, Cat. # K1012050)
  • QIAamp Circulating Nucleic Acid Kit (Qiagen, Cat. # 55114)
  • cfKapture Kit (Magbio)
  • QIAamp MinElute ccfDNA Mini Kit (Qiagen)
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Automated Extraction

  • (Maxwell® RSC Blood DNA Kit, Promega, Cat.# AS1400)
  • (Agencourt Genfind v2, Beckman Coulter, Cat. # A41501, A41499, A41497, A41500)
  • AnaPrep 12 Blood DNA Extraction Kit (BioChain, Ct. # z1322001)

 

iSWAB automated liquid handling compatible platforms

  • Perkin Elmer: chemagic MSM I
  • Beckman Coulter: Biomek, SPRI-TE
  • Hamilton Star, Hamilton Nimbus
  • Tecan Evo
  • Promega Maxwell-16 RSC, or RSC 48