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iSWAB™-Microbiome Collection Kit
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iSWAB™-Microbiome Collection Device Rack
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Benefits

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Microbial content and diversity within collected biosamples can provide a wealth of clues about human and animal health. However, current microbiome collection methods subject the samples to harsh and stressful stabilization techniques such as freezing or harsh organic solvents. Both stabilization approaches have a high probability of altering the microbial representation in the sample, which could offer misleading results and hinder proper modeling or data analysis. Freezing the collected sample requires well-monitored cold chain storage and transport, increasing associated costs. Transport alone may alter the microbial representation from point of collection to processing. Thawing samples requires pre-processing which affects DNA quality and somewhat limits re-culturing of samples, therefore affecting proper modeling or data analysis. The use of toxic organic solvents requires strict regulations for transport and handling procedures, they also alter microbial representation from the point of collection to processing, limiting DNA and RNA analysis. The iSWAB-MB offers a non-toxic, non-organic solvent, free from hazardous fixatives and detergents, allowing the microbial representation to remain intact from the time of collection to several weeks at room temperature (real time testing ongoing).

Product detail

Features

  • Non-toxic stabilization; no organic, hazardous solvent fixatives or detergents.
  • Microbial representation maintains intact from the time of collection for weeks at room temperature (real-time testing ongoing).
  • No bloom effect correction is needed for microbiome data. Both aerobic and anaerobic bacterial communities stabilized from collection to processing.
  • Ambient temperature transport and storage with no cold chain involvement.
  • The stabilization buffer effectively inactivates bacteria, fungi, and spores allowing safe transport of biological samples.
  • Purified microbial/viral DNA or RNA isolated from collected samples are high-quality and compatible with qPCR, microarray and NGS-based applications.
  • Scalable and customizable platform capable of stabilizing up to 20g of fecal or soil material in standard collection containers.
  • Swab-free transport allows for a smaller transportation and in-lab storage footprint.

iSWAB®-Microbiome Collection Device Bag

Applications

Product Common Name: iSWAB®-MB Bag

Product Full Name: iSWAB®-Microbiome Collection Device Bag

Part Number: ISM-T-1200

Units: 1.0mL x 500 units

Includes:
500 iSWAB®-MB Devices
Clear Bag
Labels

*The products shown contain the CE Mark and are approved for IVD use in countries recognizing this standard. In the USA, products are designated For Research Use Only and not for use in diagnostic procedures and will be labeled accordingly.

Devices only for collecting microbial/viral DNA and RNA from oral, nasal, soil, skin, and fecal samples. Microbiome analysis (from Fecal, skin, vaginal, soil, etc.) qPCR, microarray, and NGS for microbiome research in health, wellness, and forensics.

Product Description

The iSWAB®-Microbiome (iSWAB®-MB) collection device consists of a collection device that is pre-filled with 1.0 mL of the iSWAB®-MB non-toxic, stabilizing buffer and fitted with a proprietary insert. The insert is designed to optimize the release of specimens collected with swabs into the stabilizing buffer, creating a minimal footprint and allowing for swab-free transport of specimens. The iSWAB®-MB buffer is designed to maintain the status quo of samples at the time of collection, providing a representative snapshot of the microbial community that remains unchanged from the point of collection to processing.

Intended Use

iSWAB®-Microbiome collection device is a non-invasive, non-toxic sample collection technology intended for stable ambient transport and storage of microbial/viral DNA and RNA from various sample types including fecal, vaginal, skin, soil, nasal, oral, and saliva. Purified microbial/viral DNA or RNA isolated from collected samples are compatible with qPCR, microarray, and NGS for microbiome research in health, wellness, and forensics.

Indication for Use (from IFU)

The iSWAB®-Microbiome sample collection technology is intended for specimen collection of biological samples. It is a non-invasive, non-toxic sample collection method and is intended to be used for long-term room temperature, stable transport, and storage of microbial/viral DNA and RNA from various sample types including fecal, vaginal, skin, soil, nasal, and oral. The iSWAB-Microbiome device is designed to recover and stabilize collected samples from a nylon flocked or molded swab head and then the swab is discarded at the point of collection. For saliva collection, only a spit funnel is needed to facilitate the collection of saliva into the iSWAB®-Microbiome device

Storage and Stability

Pre-collection: 18 months from date of manufacture between 15-30° C. Transport temperature, post-collection: 15-30° C.

Post-collection: 21 days for microbial RNA and 40 days for microbial DNA at 15-30° C; if longer storage time is required, collected samples can be stored at -20 to -80° C.

Resources

Product
ISM-T-1200
Pre-Collection
Time Temp (C)
18 mo. DOM 15-30
18 mo. DOM 15-30
Transport
Temp (C)
15-30
15-30
Analyte
Type
Microbial DNA
Microbial RNA
Post Collection
Time Temp (C)
40 Days 15-30
21 Days 15-30
Cryo Storage
Cryo Storage
-20- to -80
-20- to -80
Attributes ISM-T-1200
Sample Oral, saliva, vaginal, nasal, rectal, fecal/gut, soil, surface, skin
Use viral/microbial DNA and RNA from various sample types
# Swabs 2 or 1
Collection Volume 1.0 mL
Nucleic Acid Yield (μg) varies
Storage and Transport temperature RT
Shelf Life Stability 18 months
Post-collection Stability 40 days (Microbial DNA), 21 days (Microbial RNA)
Capability to concentrate sample Yes
Suitable for all population segments Yes
Automation friendly Yes
Liquid Sample Yes
Barcoded Included
Applications Application: Characterize skin, gut, vaginal, oral microbiome composition and their expression profiles.
Compatible Extraction Methods ZymoBiomics DNA/RNA MiniPrep Kit (Cat. # R2002, Zymo Research), QIAamp Fast DNA Stool Mini Kit (Qiagen Cat# 51604), Quick-DNA Fecal/Soil Microbe Miniprep Kit (Zymo Research Cat# 6010), DNeasy PowerSoil Pro Kit (Qiagen Cat#47014), QIAamp PowerFecal Pro DNA Kit (Qiagen, Cat #51804), MagAttract PowerMicrobiome DNA/RNA EP (Qiagen, Cat#27500), NucleoMag DNA Microbiome (Macherey-Nagel, Cat# 67-63-0), RNeasy PowerFecal Pro kit (Qiagen, Cat#78404)
Downstream Assays Metagenomic sequencing, metatranscriptomic sequencing, qPCR, RT-qPCR

WARNING AND PRECAUTIONS

For external use and single use only. DO NOT drink, touch, or remove the buffer from the device. Do Not use if device is broken, leaking, or visibly damaged. These products should only be used in accordance with the instructions provided. Avoid eye contact with the liquid in the device. IF IN EYES: Rinse cautiously with water for several minutes. IF INGESTED: Call POISON CENTER. Rinse mouth. Not a Hazardous Combustion Product. Device cap can be a choking hazard. Do not ingest. Refer to SDS.