Benefits

Maintaining RNA stability and protecting it from degradation is a significant challenge, while current RNA stabilization methods such as PAXgene, Tempus, RNAgard or dry blood spots require invasive blood collection resulting in low compliance. The need to collect blood results in a very limited shelf life, forcing labs to invest in cold storage infrastructure or commit resources to immediate sample processing. Other options for non-invasive collection also suffer from short shelf life storage as well as low quality and unusable RNA. Mawi DNA Technologies developed the iSWAB™- RNA-v2, the only non-invasive technology for the collection and extended room temperature stabilization of high-quality RNA from oral samples with applications in microarray and sequencing-based gene expression profiling and transcriptomics.

Product detail

Features

Swab-free sample transport: Decrease sample processing time without compromising sample integrity.
Increase process efficiency: Extended shelf life stability (up to 4 weeks at room temp, several years at -20 C and -80 C), reduced processing time, and minimal storage footprint.
High-quality RNA for downstream applications such as RT-PCR, Microarray and RNA-seq for gene expression analysis.
Room temperature stable: Reduce sample storage and transport costs by eliminating cold chain requirements.
Self-collection or assisted collection in less than 2 minutes: Suitable for all population segments including infants, toddlers, and the elderly.
Traceable and reliable chain of custody: LIMS-compatible unique barcodes included on each collection device for efficient traceability and storage purposes.
Scalable and easy to process: Manual and automation-friendly sample processing.

iSWAB™-RNA-v2 Collection Device Bag

Applications

Product Common Name: iSWAB™-RNA-v2 Bag

Product Full Name: iSWAB™-RNA-v2 Collection Device Bag

Part Number: ISR2-T-1200

Units: 1.0 mL

Includes:
500 iSWAB™-RNA-v2 Devices
Clear Bag
Labels

*The products shown contain the CE Mark and are approved for IVD use in countries recognizing this standard. In the USA, products are designated For Research Use Only and not for use in diagnostic procedures and will be labeled accordingly.

Devices only for collecting human RNA samples via buccal cells.
Transcriptomics, Gene Expression: qPCR and RNA-based sequencing (RNAseq) , microarray, Norther Blot, Nuclease protection assays, cDNA library construction, Mass spectrometry, ELISA, Western blotting, tissue biopsy stabilization.

Product Description

The iSWAB™-RNA-v2 device is a non-invasive technology for the collection, concentration, and stabilization of intact buccal cells or any mammalian cells collected with a swab or cytobrush allowing for real-time ambient stabilization of total RNA from the point of collection to processing. The design of the iSWAB™ collection device allows for the maximum release of collected samples from multiple swabs or brushes into a single device, to achieve a more concentrated sample. Self-collection or assisted collection can be achieved in less than 2 minutes and is suitable for all population segments including infants, toddlers, and elderlies.

Intended Use

iSWAB™-RNA-v2 Collection Kit is a RNA sample collection device for the collection, stabilization, concentration, transport and storage of RNA samples. It is a non-invasive swab-based sampling applicator and is intended to be used for sample collection from various sample types including intact buccal cells and/or any mammalian cells collected with a swab or cytobrush.

Indication for Use (from IFU)

The iSWAB™-RNA-v2 device allows for the non-invasive collection, concentration, and stabilization of intact buccal cells and/or any mammalian cells collected with a swab or cytobrush. Allows for real time ambient stabilization of total RNA from the point of collection to processing. The iSWAB™-RNA-v2 maintains RNA stability and protects RNA from degradation. The device allows extended shelf life stability (up to 4 weeks at room temp, several years at -20 C and -80 C), reduced processing time, and minimal storage footprint.

Storage and Stability

Pre-collection: 18 months from date of manufacture between 15-30° C.
Transport temperature, post-collection: 15-30° C.
Sample stability and storage, post-collection: viable cells are stabilized for up to 4 weeks at at 15-30° C; if longer storage time is required, collected samples can be stored at -20 to -80° C for several years.

Attributes	iSWAB™-RNA-v2
Sample	Buccal
Use	Mammalian RNA
# Swabs	4
Collection Volume	1.0 mL
Nucleic Acid Yield (μg)	varies**
Storage and Transport temperature	RT
Shelf Life Stability	18 months
Post-collection Stability	4 weeks
Capability to concentrate sample	Yes
Suitable for all population segments	Yes
Automation friendly	Yes
Liquid Sample	Yes
Barcoded	Included
Compatible Assays	Illumina Stranded Total RNA Prep w/ Ribo-Zero (Illumina, Cat. # 20040525, 20040529), Human EGFR In-Cell ELISA Kit (abcam, Cat. # ab126419)
Applications	Illumina Stranded Total RNA Prep w/ Ribo-Zero (Illumina, Cat. # 20040525, 20040529), Human EGFR In-Cell ELISA Kit (abcam, Cat. # ab126419)

Other presentations

iSWAB™-RNA-v2 Collection Kit

iSWAB™-RNA-v2 Collection Device Rack