What It's Used For
During the height of the COVID-19 pandemic, viral testing became the most effective tool to help bring outbreaks and surges under control. However, with new COVID variants on the rise and ongoing supply chain shortage for viral extraction reagents, Mawi DNA directed their focus to develop a technology that would decrease processing time for COVID testing.
Mawi DNA Technologies has developed a modified “extraction-less” version of the iSWAB technology. The ISWAB-Microbiome-EL is designed to eliminate the RNA extraction step in the COVID-19 Molecular testing workflow, allowing researchers to perform direct RT-PCR on individual and pooled samples.
The iSWAB-Microbiome (MB) is a non-toxic stabilizing technology that enables the inactivation of bacteria, fungi, spores, and viruses, allows ambient collection and transport of various biosamples, and maintains the status quo at the time of collection. iSWAB-MB-EL is compatible and can stabilize both nasal swabs and saliva samples, and these samples can be used directly in PCR reactions without any prior major (RNA extraction) or minor (heating or/and Proteinase K treatment) sample processing and successfully detect SARS-CoV-2 without any observed PCR inhibition.
iSWAB- Microbiome-EL is compatible with both nasal and saliva collection, inactivates SARS-CoV-2 for safe collection and storage iSWAB-Microbiome-EL, and has been specifically designed to eliminate the RNA extraction step in the COVID-19 testing workflow; allowing researchers to perform direct RT-PCR on individual and pooled samples.
- Extraction-less, extraction-free PCR
- Reduce processing time by eliminating the extraction step
- Efficiently inactivates SARS-CoV-2 virus and other bacteria, fungi, spores, and viruses to allow safe transport of biological samples.
- Diminish risk of infection during sample collection process
- Long term room temperature stabilization up to 21 days
- Compatible with any nasal and or/ saliva collection swabs
- Non-toxic stabilization; upholds the integrity of sample with no degradation
- Barcoded for sample traceability
- Proven Sensitivity & Testing accuracy
- LAMP Assays
- Bio-RAD Reliance SARs CoV-2 RT Assays Kit, Cat. #12014115 (EUA Granted)
- Prime Discoveries: Prime COVID-19 Extraction Less High Throughput LAMP Assay Kit (EUA Pending)
- SeqOnce Bio AzureSeq Direct One Step Universal RT-qPCR Kit SARS CoV-2, Ca# ASD-200 (EUA-Validated, not EUA- Authorized)
- 3CR Bio: ProbeSure COVID-19 One Step RT-PCR Kit, CA #COV 1001-3
- Takara: One Step PrimeScript III RT-PCR Kit, Cat. no. RR600A, RR600S, RR600B