Microbial content and diversity within collected gut and rectal samples can provide a wealth of clues about human and animal health. However, current microbiome collection methods subject the samples to harsh and stressful stabilization techniques such as freezing or harsh organic solvents.
Transport alone may alter the microbial representation from point of collection to processing. Thawing samples requires pre-processing which affects DNA quality and somewhat limits re-culturing of samples, therefore affecting proper modeling or data analysis.
Both stabilization approaches have a high probability of altering the microbial representation in the sample, which could offer misleading results and hinder proper modeling or data analysis. Freezing the collected sample requires well-monitored cold chain storage and transport, increasing associated costs.
The use of toxic organic solvents requires strict regulations for transport and handling procedures, they also alter microbial representation from the point of collection to processing, limiting DNA and RNA analysis. The iSWAB-MB offers a non-toxic, non-organic solvent, free from hazardous fixatives and detergents, allowing the microbial representation to remain intact from the time of collection to several weeks at room temperature (real time testing ongoing).
Non-toxic stabilization; no organic, hazardous solvent fixatives or detergents. Microbial representation maintained intact from time of collection for weeks at room temperature (real time testing ongoing). No bloom effect correction needed for microbiome data. Both aerobic and anaerobic bacterial communities stabilized from collection to processing. Ambient temperature transport and storage with no cold chain involvement.
The stabilization buffer effectively inactivates bacteria, fungi, and spores allowing safe transport of biological samples. Purified microbial DNA or RNA isolated from collected samples are high-quality and compatible with qPCR, microarray and NGS based applications. Scalable and customizable platform capable of stabilizing up to 20g of fecal or soil material in standard collection containers. Swab-free transport allows for a smaller transportation and in-lab storage footprint.
Microbiome analysis (from Fecal, skin, vaginal, soil, etc.) qPCR, microarray, and NGS for microbiome research in health, wellness, and forensics.
The iSWAB™-Microbiome (iSWAB™-MB) collection device consists of a collection device that is pre-filled with 1.0 mL of the iSWAB™-MB non-toxic, stabilizing buffer and fitted with aproprietary insert. The insert is designed to optimize the release of specimens collected with swabs into the stabilizing buffer, creating a minimal footprint and allowing for swab-free transport of specimens. The iSWAB™-MB buffer is designed to maintain the status quo of samples at the time of collection, providing a representative snapshot of the microbial community that remains unchanged from the point of collection to processing.
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iSWAB™-Microbiome collection device is a non-invasive, non-toxic sample collection technology intended for stable ambient transport and storage of microbial DNA and RNA from various sample types including fecal, vaginal, skin, soil, nasal, oral, and saliva. Purified microbial DNA or RNA isolated from collected samples are compatible with qPCR, microarray, and NGS for microbiome research in health, wellness, and forensics.
The iSWAB™-Microbiome collection device is intended for the stabilization and inactivation of upper and lower respiratory human specimens suspected of containing SARS-CoV-2 and other biosamples with microbial communities. This device can be used for the collection, transport, and storage of SARS-CoV-2 specimens at ambient temperature (15-30 °C) for 42 days and allows for the transportation of samples without a swab. The RNA extracted from the collected samples is suitable for use with legally marketed molecular diagnostic assays.
Pre-collection: 18 months from date of manufacture between 15-30° C. Transport temperature, post- collection: 15-30° C. Sample stability and storage, post-collection: 42 days for SARS-CoV-2 samples, 21 days for microbial RNA and 40 days for microbial DNA at 15-30° C; if longer storage time is required, collected samples can be stored at -20 to -80° C. Additional information available at www.mawidna.com.
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| Attributes | iSWAB-DNA-1200 |
|---|---|
| Sample | Buccal |
| Use | Mammalian DNA |
| # Swabs | 4 |
| Collection Volume | 1000 µl |
| Nucleic Acid Yield (μg) | 10-30 |
| Molecular Weight of DNA | 23 kb |
| Bacterial DNA content | <1% |
| Storage and Transport temperature | RT |
| Shelf Life Stability | 3 years |
| Post-collection Stability | Months |
| Capability to concentrate sample | Yes |
| Suitable for all population segments | Yes |
| Automation friendly | Yes |
| Liquid Sample | Yes |
| Barcoded | Included |
| Compatible Assays | Investigator Quantiplex Kit (Qiagen, Cat. # 387016), Promega PowerpPlex 16 HS System (Promega, Cat. # DC2101), EZ1 Advanced XL DNA Investigator Flip-Cap Card (Qiagen, Cat. # 9019933), TaqMan Open Array Genotyping assays |
| Applications | Population Genomics, Cancer research HLA typing, DNA sequencing; Sanger and Next generation sequencing, Epidemiology |
For external use and single use only. DO NOT drink, touch, or remove the buffer from the device. Do Not use if device is broken, leaking, or visibly damaged. These products should only be used in accordance with the instructions provided. Avoid eye contact with the liquid in the device. IF IN EYES: Rinse cautiously with water for several minutes. If Ingested Call a POISON CENTER. Rinse mouth. Not a Hazardous Combustion Product. Device cap can be a choking hazard. Do not ingest.
