COVID 19 Molecular Testing
-Tempus EUA
–Avellino Labs EUA
–Warrior Dx EUA
–Helix EUA (RT-PCR)
–Helix EUA (NGS)
Sample Collection from Infants using iSWAB
– Paper: Cohort profile: Understanding Pregnancy Signals and Infant Development (UPSIDE): a pregnancy cohort study on prenatal exposure mechanisms for child health
Molecular Analysis of Microbial DNA/RNA
– Paper: Detection Of A Negative Biomarker For Amebiasis (Entamoeba Histolytica) In The Human Gut Mycobiome
– AACR Poster Abstract A25: Collecting gut microbiome in the 10,000 Families Study
– Microbiome Signature and Diversity Profiling of Normal Skin of Human in Saudi Arabia
Wide Temperature ranges (-80°C to 65°C) Long Term DNA Stability for Transport and Storage
– Maximizing Forensics DNA Recovery & Long-term Room Temperature Stabilization (PPT)
– Maximizing the amount of DNA recovered; a study of Mawi DNA Technologies’ iSWAB-ID collection device for forensic science application (Master Thesis 2017)
– iSWAB Enables Forensic Efficient DNA Recovery & Storage
– An Efficient Non-Invasive Sample Collection Technology for Various Population Segments, Qatar Foundation Annual Research Conference, At Doha, Qatar, Volume: Second 2014
High Quality RNA Extraction
– Extracting high quality RNA from iSWAB-RNA-v2
Performance of different swab material with iSWAB
– Performance Comparison of CopanFLOQ with other types of swabs w/iSWAB
– Longer gDNA Stability, Lower Bacterial DNA Contamination, and Greater Cost Efficiency; The Choice is Yours
Buccal Cells, not Saliva
– 20% – 46% of the Population Suffers from Dry Mouth: Saliva Collection is not a Universal Sample Collection Tool
– Invasive vs Non-Invasive – DNA quality downstream
– Conference: Qatar Foundation Annual Research Conference, At Doha, Qatar, Volume: Second, 2014
Next Generation Sequencing
– Invasive vs Non-Invasive – DNA quality downstream
– iSWAB Poster Epigenomics 2016
– iSWAB-DNA: Whole Exome Sequencing approved
– De novo variants in GREB1L unveiled with Exome sequencing from buccal cells
– A de novo SIX1 variant in a patient with a rare nonsyndromic cochleovestibular nerve abnormality, cochlear hypoplasia, and bilateral sensorineural hearing loss – (2019)
Whole Exome Sequencing
– Exome Sequencing of Two Siblings with Sporadic Autism Spectrum Disorder and Severe Speech Sound Disorder Suggests Pleiotropic and Complex Effects – (2019)
– Evaluation of TGFBI corneal dystrophy and molecular diagnostic testing – (2019)
– Trauma-induced exacerbation of epithelial-stromal TGFBI lattice corneal dystrophy – (2018)
– De novo variants unveil inner ear malformation and deafness – (2018)
– A de novo SIX1 variant in a patient with a rare nonsyndromic cochleovestibular nerve abnormality, cochlear hypoplasia, and bilateral sensorineural hearing loss – (2019)
Epigenomics and Methylation
– Paper: Early-life environment programs reproductive strategies through epigenetic regulation of SRD5A1
– Paper: Epigenetic signatures of attachment insecurity and childhood adversity provide evidence for role transition in the pathogenesis of perinatal depression
– The EMPHASIS study
– Intergrowth 21st
– Deep clinical and biological phenotyping of the preterm birth and small gestational age syndromes: The INTERBIO-21st Newborn Case-Control Study protocol
– Selective Lysis Enables Effective Whole Genome Bisulphite Sequencing of Buccal
Epithelial Samples
– Epigenetic Differences in Inflammation Genes of Monozygotic Twins are related to Parental Emotional Availability and Health
-Paper: Harsh Parenting Predicts Novel HPA Receptor Gene Methylation and NR3C1 Methylation Predicts Cortisol Daily Slope in Middle Childhood
Forensics
– Maximizing Forensics DNA Recovery & Long-term Room Temperature Stabilization (PPT)
– Maximizing the amount of DNA recovered: a study of Mawi DNA Technologies’ iSWAB-ID collection device for forensic science application (Master Thesis 2017)
– iSWAB Enables Forensic Efficient DNA Recovery & Storage
– Superior Forensic STR Profiling with iSWAB-ID
PCR
Mawi Sample Prep for Direct PCR or RT-PCR
Proteomics
– Detection of Frataxin using iSWAB-Protein UAB
– Paper: Detection of Oxidative Stress in Buccal Cells using iSWAB Tubes
Animal Speciation and Genetics
– iSWAB Performance in Animal Speciation
Automation Compatibility
– High-Yield Automated gDNA Isolation using Perkin Elmer chemagic Technology
– Extraction automation on Hamilton Star with Omega Purification Kit (video)
– Automated Extraction of iSWAB with Omega and Hamilton
– Extraction of iSWABs with SPRI
– Biomek Compatibility Guide
– Promega: DNA Purification from iSWAB-Discovery on Promega Maxwell Platform
– Qiagen QiaSymphony (Custom method # B400_CR21913, Contact Qiagen Apps team)
OMICS From The Same Sample
– OMICS Enabled with iSWAB-Cells all from the same sample
– Waterpipe Smoke Effect on Oxidative Stress Levels in Buccal Cells Using Mawi iSWAB-Protein Collection Tubes
– A pilot study to investigate the feasibility of transporting saliva samples at room temperature with MAWI Cell Stabilization buffer