COVID 19 Molecular Testing
– UCSD EUA Extraction-Less
– UCSD EUA (suitable for at-home collection)
– Tempus EUA
– Avellino Labs EUA
– Warrior Dx EUA
– Helix EUA (RT-PCR)
– Helix EUA (NGS)
– Perkin Elmer EUA
Sample Collection from Infants using iSWAB
– Paper: Cohort profile: Understanding Pregnancy Signals and Infant Development (UPSIDE): a pregnancy cohort study on prenatal exposure mechanisms for child health
Molecular Analysis of Microbial DNA/RNA
Nasal
-High Sensitivity, Rapid Detection of Virus in High Traffic Environments
–A rapid, specific, extraction-less, and cost-effective RT-LAMP test for the detection of SARS-CoV-2 in clinical specimens
Oral
–Sterilization effects of commercial denture cleaners compared with a combination of denture cleaners and ultrasonic cleaning
Fecal
– Paper Detection Of A Negative Biomarker For Amebiasis (Entamoeba Histolytica) In The Human Gut Micobiome
– Attributes of intestinal microbiota compositionand their correlation with clinical primary nonresponse to anti-TNF-α agents ininflammatory bowel disease patients
– AACR Poster Abstract A25: Collecting gut microbiome in the 10,000 Families Study
-Paper Genetic and Gut Microbiome Signals for the Management of Functional Gastrointestinal Disorders
-Poster Use of Novel Non-Toxic Sample Collection Technology for Accurate Microbial Community Profiling through Long-Term Stabilization of Microbiome samples at Ambient Temperature
Vaginal
-Development of qPCR Assay for Genital Microbial Signatures
Skin
– Microbiome Signature and Diversity Profiling of Normal Skin of Human in Saudi Arabia
Wide Temperature ranges (-80°C to 65°C) Long Term DNA Stability for Transport and Storage
– Maximizing Forensics DNA Recovery & Long-term Room Temperature Stabilization (PPT)
– Maximizing the amount of DNA recovered; a study of Mawi DNA Technologies’ iSWAB-ID collection device for forensic science application (Master Thesis 2017)
– iSWAB Enables Forensic Efficient DNA Recovery & Storage
– An Efficient Non-Invasive Sample Collection Technology for Various Population Segments, Qatar Foundation Annual Research Conference, At Doha, Qatar, Volume: Second 2014
High Quality RNA Extraction
– Extracting high quality RNA from iSWAB-RNA-v2
Performance of different swab material with iSWAB
– Performance Comparison of CopanFLOQ with other types of swabs w/iSWAB
– Longer gDNA Stability, Lower Bacterial DNA Contamination, and Greater Cost Efficiency; The Choice is Yours
Buccal Cells, not Saliva
– 20% – 46% of the Population Suffers from Dry Mouth: Saliva Collection is not a Universal Sample Collection Tool
– Invasive vs Non-Invasive – DNA quality downstream
– Conference: Qatar Foundation Annual Research Conference, At Doha, Qatar, Volume: Second, 2014
Next Generation Sequencing
– Invasive vs Non-Invasive – DNA quality downstream
– iSWAB Poster Epigenomics 2016
– iSWAB-DNA: Whole Exome Sequencing approved
– De novo variants in GREB1L unveiled with Exome sequencing from buccal cells
– A de novo SIX1 variant in a patient with a rare nonsyndromic cochleovestibular nerve abnormality, cochlear hypoplasia, and bilateral sensorineural hearing loss – (2019)
Whole Exome Sequencing
– Paper: Genetic prescreening of a candidate for laser refractive surgery identifies risk for inadequate tissue response: a case report
– Exome Sequencing of Two Siblings with Sporadic Autism Spectrum Disorder and Severe Speech Sound Disorder Suggests Pleiotropic and Complex Effects – (2019)
– Evaluation of TGFBI corneal dystrophy and molecular diagnostic testing – (2019)
– Trauma-induced exacerbation of epithelial-stromal TGFBI lattice corneal dystrophy – (2018)
– De novo variants unveil inner ear malformation and deafness – (2018)
– A de novo SIX1 variant in a patient with a rare nonsyndromic cochleovestibular nerve abnormality, cochlear hypoplasia, and bilateral sensorineural hearing loss – (2019)
Epigenomics and Methylation
– Paper: Maternal attachment insecurity, maltreatment history, and depressive symptoms are associated with broad DNA methylation signatures in infants
– Paper: Epigenetic regulation of 5α reductase -1 underlies adaptive plasticity of reproductive function and pubertal timing
– Paper: Early-life environment programs reproductive strategies through epigenetic regulation of SRD5A1
– Paper: Epigenetic signatures of attachment insecurity and childhood adversity provide evidence for role transition in the pathogenesis of perinatal depression
– The EMPHASIS study
– Intergrowth 21st
– Deep clinical and biological phenotyping of the preterm birth and small gestational age syndromes: The INTERBIO-21st Newborn Case-Control Study protocol
– Selective Lysis Enables Effective Whole Genome Bisulphite Sequencing of Buccal Epithelial Samples
– Epigenetic Differences in Inflammation Genes of Monozygotic Twins are related to Parental Emotional Availability and Health
-Paper: Harsh Parenting Predicts Novel HPA Receptor Gene Methylation and NR3C1 Methylation Predicts Cortisol Daily Slope in Middle Childhood
Forensics
– Maximizing Forensics DNA Recovery & Long-term Room Temperature Stabilization (PPT)
– Maximizing the amount of DNA recovered: a study of Mawi DNA Technologies’ iSWAB-ID collection device for forensic science application (Master Thesis 2017)
– iSWAB Enables Forensic Efficient DNA Recovery & Storage
– Superior Forensic STR Profiling with iSWAB-ID
-Development of qPCR Assay for Genital Microbial Signatures
– Copan AAFS iSWAB FLOQSWABS
PCR
Mawi Sample Prep for Direct PCR or RT-PCR
iSWAB-RNA-v2 in extraction-less RT-PCR
Proteomics
– Detection of Frataxin using iSWAB-Protein UAB
– Paper: Detection of Oxidative Stress in Buccal Cells using iSWAB Tubes
Animal Speciation and Genetics
– iSWAB Performance in Animal Speciation
Automation Compatibility
– High-Yield Automated gDNA Isolation using Perkin Elmer chemagic Technology
– Extraction automation on Hamilton Star with Omega Purification Kit (video)
– Automated Extraction of iSWAB with Omega and Hamilton
– Extraction of iSWABs with SPRI
– Biomek Compatibility Guide
– Promega: DNA Purification from iSWAB-Discovery on Promega Maxwell Platform
– Qiagen QiaSymphony (Custom method # B400_CR21913, Contact Qiagen Apps team)
OMICS From The Same Sample
– OMICS Enabled with iSWAB-Cells all from the same sample
– Waterpipe Smoke Effect on Oxidative Stress Levels in Buccal Cells Using Mawi iSWAB-Protein Collection Tubes
– A pilot study to investigate the feasibility of transporting saliva samples at room temperature with MAWI Cell Stabilization buffer